|Application ||IF, WB, E|
|Other Accession||Q9QY01, NP_001136082.1|
|Calculated MW||112694 Da|
|Antigen Region||300-327 aa|
|Other Names||Serine/threonine-protein kinase ULK2, Unc-51-like kinase 2, ULK2, KIAA0623|
|Target/Specificity||This ULK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-327 amino acids from the Central region of human ULK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ULK2 Antibody (Center S323) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.|
|Cellular Location||Cytoplasmic vesicle membrane; Peripheral membrane protein. Note=Localizes to pre- autophagosomal membrane|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq].
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Jung, C.H., et al. Mol. Biol. Cell 20(7):1992-2003(2009)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Tomoda, T., et al. Genes Dev. 18(5):541-558(2004)
Yan, J., et al. Oncogene 18(43):5850-5859(1999)
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