|Application ||WB, E|
|Other Accession||O35593, NP_005796.1|
|Calculated MW||34577 Da|
|Antigen Region||258-287 aa|
|Other Names||26S proteasome non-ATPase regulatory subunit 14, 3419-, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1, PSMD14, POH1|
|Target/Specificity||This PSMD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from the C-terminal region of human PSMD14.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PSMD14 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non- homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.|
|Tissue Location||Widely expressed. Highest levels in heart and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
PSMD14 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway (Spataro et al., 1997 [PubMed 9374539]).
Byrne, A., et al. Exp. Cell Res. 316(2):258-271(2010)
Ma, Z., et al. Pediatr Int 51(5):732-735(2009)
Cooper, E.M., et al. EMBO J. 28(6):621-631(2009)
Koulich, E., et al. Mol. Biol. Cell 19(3):1072-1082(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
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