|Application ||WB, E|
|Calculated MW||66510 Da|
|Antigen Region||446-475 aa|
|Other Names||Protein enabled homolog, ENAH, MENA|
|Target/Specificity||This ENAH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-475 amino acids from the C-terminal region of human ENAH.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ENAH Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, filopodium. Cell junction, synapse. Cell junction, focal adhesion. Note=Targeted to the leading edge of lamellipodia and filopodia by MRL family members Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses (By similarity).|
|Tissue Location||Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions|
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Provided below are standard protocols that you may find useful for product applications.
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes (By similarity).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Chattopadhyay, I., et al. Mutat. Res. 696(2):130-138(2010)
Hahn, W.H., et al. Exp. Mol. Med. 41(11):793-801(2009)
Gurzu, S., et al. Rom J Morphol Embryol 50(2):213-216(2009)
Higashi, M., et al. PLoS ONE 4 (3), E4765 (2009) :
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