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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IsocitRe dehydrogenase (IDH3) Antibody (C-term)   

IsocitRe dehydrogenase (IDH3) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - IsocitRe dehydrogenase (IDH3) Antibody (C-term) AP1927A
    Western blot analysis of Isocitrate dehydrogenase (Cat. #AP1927a) in MCF-7, Jurkat cell line lysates (35ug/lane). Isocitrate dehydrogenase (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P50213
Other Accession Q99NA5, Q9D6R2, Q28480, P41563
Reactivity Human
Predicted Bovine, Monkey, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39592 Da
Antigen Region 317-346 aa
Additional Information
Gene ID 3419
Other Names Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, Isocitric dehydrogenase subunit alpha, NAD(+)-specific ICDH subunit alpha, IDH3A
Target/Specificity This Isocitrate dehydrogenase (IDH3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human Isocitrate dehydrogenase (IDH3).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIsocitRe dehydrogenase (IDH3) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IDH3A
Cellular Location Mitochondrion.
Research Areas
Citations ( 0 )


Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.


Soundar, S., et al., J. Biol. Chem. 278(52):52146-52153 (2003).
Weiss, C., et al., Biochemistry 39(7):1807-1816 (2000).
Kim, Y.O., et al., J. Biol. Chem. 274(52):36866-36875 (1999).
Huh, T.L., et al., Genomics 32(2):295-296 (1996).
Kim, Y.O., et al., Biochem. J. 308 (PT 1), 63-68 (1995) (): ().

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$ 295.00
$ 99.00
Cat# AP1927A
(40 western blots)
Availability: In Stock
Bulk Size
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