|Application ||WB, E|
|Calculated MW||119652 Da|
|Antigen Region||955-983 aa|
|Other Names||E3 ubiquitin-protein ligase RNF31, 632-, HOIL-1-interacting protein, HOIP, RING finger protein 31, Zinc in-between-RING-finger ubiquitin-associated domain protein, RNF31, ZIBRA|
|Target/Specificity||This RNF31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-983 amino acids from the C-terminal region of human RNF31.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RNF31 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF- induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types.|
|Tissue Location||Expressed in both normal and transformed breast epithelial cell lines.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.
Silva, L.K., et al. Eur. J. Hum. Genet. (2010) In press : Ehrlund, A., et al. Mol. Cell. Biol. 29(8):2230-2242(2009) Tokunaga, F., et al. Nat. Cell Biol. 11(2):123-132(2009) Kirisako, T., et al. EMBO J. 25(20):4877-4887(2006) Lim, J., et al. Cell 125(4):801-814(2006)
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