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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RNF31 Antibody (C-term)   

RNF31 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RNF31 Antibody (C-term) AP19285b
    All lanes : Anti-RNF31 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RNF31 Antibody (C-term) AP19285b
    All lanes : Anti-RNF31 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RNF31 Antibody (C-term) AP19285b
    All lanes : Anti-RNF31 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 120 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RNF31 Antibody (C-term) AP19285b
    RNF31 Antibody (C-term)(Cat. #AP19285b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RNF31 antibody detected the RNF31 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96EP0
Other Accession NP_060469.4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 119652 Da
Antigen Region 955-983 aa
Additional Information
Gene ID 55072
Other Names E3 ubiquitin-protein ligase RNF31, 632-, HOIL-1-interacting protein, HOIP, RING finger protein 31, Zinc in-between-RING-finger ubiquitin-associated domain protein, RNF31, ZIBRA
Target/Specificity This RNF31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-983 amino acids from the C-terminal region of human RNF31.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF31 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF31
Synonyms ZIBRA
Function E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF- induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types.
Cellular Location Cytoplasm.
Tissue Location Expressed in both normal and transformed breast epithelial cell lines.
Research Areas
Citations (0)

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Background

The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.

References

Silva, L.K., et al. Eur. J. Hum. Genet. (2010) In press : Ehrlund, A., et al. Mol. Cell. Biol. 29(8):2230-2242(2009) Tokunaga, F., et al. Nat. Cell Biol. 11(2):123-132(2009) Kirisako, T., et al. EMBO J. 25(20):4877-4887(2006) Lim, J., et al. Cell 125(4):801-814(2006)

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$ 295.00
$ 99.00
Cat# AP19285b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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