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PMF1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PMF1 Antibody (Center) AP19290c
    All lanes : Anti-PMF1 Antibody (Center) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q6P1K2
Other Accession NP_009152.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23339 Da
Antigen Region 109-135 aa
Additional Information
Gene ID 100527963;11243
Other Names Polyamine-modulated factor 1, PMF-1, PMF1 {ECO:0000312|EMBL:AAH650311}
Target/Specificity This PMF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-135 amino acids from the Central region of human PMF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPMF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PMF1 {ECO:0000312|EMBL:AAH65031.1}
Function Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
Cellular Location Nucleus. Chromosome, centromere, kinetochore Note=Associated with the kinetochore
Tissue Location Highest levels of expression in heart and skeletal muscle, with significant levels expressed in kidney and liver.
Research Areas
Citations (0)

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Background

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.

References

Aleman, A., et al. Clin. Cancer Res. 14(24):8236-8243(2008)
Kline, S.L., et al. J. Cell Biol. 173(1):9-17(2006)
Obuse, C., et al. Nat. Cell Biol. 6(11):1135-1141(2004)
Wang, Y., et al. Biochem. J. 366 (PT 1), 79-86 (2002) :
Wang, Y., et al. Biochem. J. 355 (PT 1), 45-49 (2001) :

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$ 295.00
$ 99.00
Cat# AP19290c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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