|Application ||WB, E|
|Other Accession||Q8BGE6, Q6DG88, Q6PZ02, Q6PZ03, NP_037457|
|Predicted||Bovine, Chicken, Zebrafish, Mouse|
|Calculated MW||44294 Da|
|Antigen Region||239-265 aa|
|Other Names||Cysteine protease ATG4B, 3422-, AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, hAPG4B, ATG4B, APG4B, AUTL1, KIAA0943|
|Target/Specificity||This APG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-265 amino acids from the Central region of human APG4B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APG4B Antibody (Center G254) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||APG4B, AUTL1, KIAA0943|
|Function||Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.|
|Tissue Location||Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
Satoo, K., et al. EMBO J. 28(9):1341-1350(2009) Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : Olsen, J.V., et al. Cell 127(3):635-648(2006) Olsen, J.V., et al. Cell 127(3):635-648(2006)
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