|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8BFY9, Q3SYU7|
|Calculated MW||102355 Da|
|Antigen Region||47-74 aa|
|Other Names||Transportin-1, Importin beta-2, Karyopherin beta-2, M9 region interaction protein, MIP, TNPO1, KPNB2, MIP1, TRN|
|Target/Specificity||This TNPO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-74 amino acids from the N-terminal region of human TNPO1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TNPO1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KPNB2, MIP1, TRN|
|Function||Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9- containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Mediates nuclear import of ADAR/ADAR1 (isoform 5) in a RanGTP-dependent manner.|
|Cellular Location||Cytoplasm. Nucleus.|
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Provided below are standard protocols that you may find useful for product applications.
TNPO1 comprises the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins.
Fineberg, K., et al., Biochemistry 42(9):2625-2633 (2003).
Nelson, L.M., et al., Virology 306(1):162-169 (2003).
Le Roux, L.G., et al., J. Virol. 77(4):2330-2337 (2003).
Limon, A., et al., J. Virol. 76(21):10598-10607 (2002).
Dvorin, J.D., et al., J. Virol. 76(23):12087-12096 (2002).
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