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ACO2 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1936a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 1
- Reviews
- Protocols
- Backgrounds
ACO2 Antibody (N-term) - Product info | |
| Application | WB
|
| Primary Accession | Q99798 |
| Other Accession | Q9ER34, P16276, Q99KI0, P20004 |
| Reactivity | Human, Rat |
| Predicted | Bovine, Mouse, Pig |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 85425 Da |
ACO2 Antibody (N-term) - Additional info | |
| Gene ID 50 | |
| Other Names ACO2; Aconitate hydratase, mitochondrial; Citrate hydro-lyase | |
| Target/Specificity This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130~160 amino acids from the N-terminal region of human Aconitase. | |
| Dilution WB~~1:100-500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACO2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACO2 Antibody (N-term) - Protein Information | |
| Name ACO2 | |
| Function Catalyzes the isomerization of citrate to isocitrate via cis-aconitate (By similarity) | |
| Cellular Location Mitochondrion (By similarity). | |
ACO2 Antibody (N-term) - Related products
AP1936a: ACO2 Antibody (N-term)
AP1936c: ACO2 Antibody (Center)
AP7561b: ACO2 Antibody (C-term)
AP7561c: ACO2 Antibody (Center)
AP7561d: ACO2 Antibody (C-term)
BP1936a: ACO2 Antibody (N-term) Blocking Peptide
BP1936c: ACO2 Antibody (Center) Blocking Peptide
BP7561b: ACO2 Antibody (C-term) Blocking Peptide
ACO2 Antibody (N-term) - Application data
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Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (N-term) (Cat# AP1936a). Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4
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ACO2 Antibody (N-term) (Cat. #AP1936a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the Aconitase antibody detected the Aconitase protein (arrow).
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ACO2 Antibody (N-term) (Cat. #AP1936a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Aconitase antibody detected the Aconitase protein (arrow).
ACO2 Antibody (N-term) - Research Areas
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BACKGROUND
ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
REFERENCES
Juang, H.H., Mol. Genet. Metab. 81(3):244-252 (2004). Bota, D.A., et al., Nat. Cell Biol. 4(9):674-680 (2002). Gruer, M.J., et al., Trends Biochem. Sci. 22(1):3-6 (1997). Klausner, R.D., et al., Mol. Biol. Cell 4(1):1-5 (1993). Geurts van Kessel, A.H., et al., Cytogenet. Cell Genet. 28(3):169-172 (1980).
