|Application ||WB, E|
|Other Accession||Q4R8H9, NP_057408.2|
|Calculated MW||74177 Da|
|Antigen Region||53-82 aa|
|Other Names||Neurotrophin receptor-interacting factor homolog, Zinc finger protein 274, Zinc finger protein HFB101, Zinc finger protein with KRAB and SCAN domains 19, Zinc finger protein zfp2, Zf2, ZNF274, ZKSCAN19|
|Target/Specificity||This ZNF274 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human ZNF274.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZNF274 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probable transcription repressor. Specifically binds to the 3'-end of zinc-finger coding genes and recruiting chromatin- modifying proteins such as SETDB1 and TRIM28/KAP1, leading to transcription repression. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).|
|Cellular Location||Cytoplasm. Nucleus, nucleolus|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals.
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Sripathy, S.P., et al. Mol. Cell. Biol. 26(22):8623-8638(2006)
Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101(44):15724-15729(2004)
Yano, K., et al. Genomics 65(1):75-80(2000)
Casademunt, E., et al. EMBO J. 18(21):6050-6061(1999)
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