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EIF3E Antibody(Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - EIF3E Antibody(Center) AP19409c
    (DANRE) eif3eb Antibody (Center) (Cat.# AP19409c) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the (DANRE) eif3eb antibody detected the (DANRE) eif3eb protein (arrow).
  • WB - EIF3E Antibody(Center) AP19409c
    EIF3E Antibody (Center)(Cat. #AP19409c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3E antibody detected the EIF3E protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P60228
Other Accession Q641X8, P60229, Q4R6G8, Q5ZLA5, Q3T102, Q3B8M3, Q1LUA8, Q05AY2, Q6DRI1, NP_001559.1
Reactivity Human, Zebrafish
Predicted Xenopus, Bovine, Chicken, Monkey, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52221 Da
Antigen Region 248-276 aa
Additional Information
Gene ID 3646
Other Names Eukaryotic translation initiation factor 3 subunit E {ECO:0000255|HAMAP-Rule:MF_03004}, eIF3e {ECO:0000255|HAMAP-Rule:MF_03004}, Eukaryotic translation initiation factor 3 subunit 6 {ECO:0000255|HAMAP-Rule:MF_03004}, Viral integration site protein INT-6 homolog, eIF-3 p48 {ECO:0000255|HAMAP-Rule:MF_03004}, EIF3E {ECO:0000255|HAMAP-Rule:MF_03004}
Target/Specificity This EIF3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the Central region of human EIF3E.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF3E Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF3E {ECO:0000255|HAMAP-Rule:MF_03004}
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF- 3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway (PubMed:17468741). May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (PubMed:17310990, PubMed:17324924).
Cellular Location Cytoplasm. Nucleus, PML body.
Tissue Location Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
Research Areas
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Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

References

Grzmil, M., et al. Oncogene 29(28):4080-4089(2010)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
Morris, C., et al. EMBO Rep. 8(6):596-602(2007)
Sirchia, R., et al. Biol. Chem. 388(5):457-465(2007)

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$ 295.00
$ 99.00
Cat# AP19409c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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