|Application ||WB, E|
|Other Accession||Q76I79, NP_061857.3|
|Calculated MW||115511 Da|
|Antigen Region||227-254 aa|
|Other Names||Protein phosphatase Slingshot homolog 1, SSH-like protein 1, SSH-1L, hSSH-1L, SSH1, KIAA1298, SSH1L|
|Target/Specificity||This SSH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-254 amino acids from the N-terminal region of human SSH1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SSH1 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cleavage furrow. Midbody. Note=Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion Also localized to the cleavage furrow and the midbody during cytokinesis|
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Provided below are standard protocols that you may find useful for product applications.
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).
Song, S.Y., et al. APMIS 118(5):389-393(2010)
Peterburs, P., et al. Cancer Res. 69(14):5634-5638(2009)
Kligys, K., et al. Biochem. Biophys. Res. Commun. 383(4):450-454(2009)
Kim, J.S., et al. Mol. Biol. Cell 20(11):2650-2660(2009)
Huang, T.Y., et al. Dev. Cell 15(5):691-703(2008)
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