Mouse Dyrk1a Antibody(C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q61214 |
Other Accession | NP_001106860.1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 85494 Da |
Antigen Region | 718-745 aa |
Gene ID | 13548 |
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Other Names | Dual specificity tyrosine-phosphorylation-regulated kinase 1A, Dual specificity YAK1-related kinase, MP86, Protein kinase minibrain homolog, MNBH, Dyrk1a, Dyrk |
Target/Specificity | This Mouse Dyrk1a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 718-745 amino acids from the C-terminal region of mouse Dyrk1a. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Mouse Dyrk1a Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Dyrk1a |
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Synonyms | Dyrk |
Function | Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities (PubMed:18938227, PubMed:20123978). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3 (By similarity). Plays an important role in double-strand breaks (DSBs) repair following DNA damage (By similarity). Mechanistically, phosphorylates RNF169 and increases its ability to block accumulation of TP53BP1 at the DSB sites thereby promoting homologous recombination repair (HRR) (By similarity). Also acts as a positive regulator of transcription by acting as a CTD kinase that mediates phosphorylation of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (By similarity). May play a role in a signaling pathway regulating nuclear functions of cell proliferation (By similarity). Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Has pro- survival function and negatively regulates the apoptotic process (PubMed:20167603). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1 (PubMed:20167603). This in turn inhibits p53/TP53 activity and apoptosis (PubMed:20167603). Phosphorylates SEPTIN4, SEPTIN5 and SF3B1 at 'Thr-434' (PubMed:18938227). |
Cellular Location | Nucleus speckle. |
Tissue Location | Detected in brain (at protein level) (PubMed:22998443). Expressed in a variety of embryonic and adult tissues (PubMed:8975710). Expressed abundantly in neurons of the brain, spinal cord, and retina in developing embryos (PubMed:8975710) Expressed in the entorhinal, temporal and visual cortices and the hippocampus of the brain where is colocalizes with SEPTIN4 (PubMed:18938227). Expressed and extensively colocalizes with SEPTIN4 in apical dendrites of pyramidal cells (PubMed:18938227). Also expressed in Purkinje cells in the cerebellum in postnatal day 1 and adult mice (PubMed:18938227). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Dyrk1a may play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates.
References
Park, J., et al. J. Biol. Chem. 285(41):31895-31906(2010)
Guo, X., et al. J. Biol. Chem. 285(17):13223-13232(2010)
Kurabayashi, N., et al. Mol. Cell. Biol. 30(7):1757-1768(2010)
Lee, Y., et al. J. Biol. Chem. 284(48):33343-33351(2009)
Noll, C., et al. PLoS ONE 4 (10), E7540 (2009) :
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