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Mouse Pbk Antibody(N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - Mouse Pbk Antibody(N-term) AP19430a
    Overlay histogram showing A431 cells stained with AP19430a (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP19430a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • IHC-P - Mouse Pbk Antibody(N-term) AP19430a
    Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody(N-term)(Cat#AP19430a). AP19430a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - Mouse Pbk Antibody(N-term) AP19430a
    Western blot analysis of lysate from Hela cell line, using Mouse Pbk Antibody (N-term)(Cat. #AP19430a). AP19430a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - Mouse Pbk Antibody(N-term) AP19430a
    Western blot analysis of lysate from mouse testis tissue lysate, using Mouse Pbk Antibody (N-term)(Cat. #AP19430a). AP19430a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9JJ78
Other Accession NP_075698.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36745 Da
Antigen Region 58-86 aa
Additional Information
Gene ID 52033
Other Names Lymphokine-activated killer T-cell-originated protein kinase, PDZ-binding kinase, T-LAK cell-originated protein kinase, Pbk, Topk
Target/Specificity This Mouse Pbk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of mouse Pbk.
Dilution FC~~1:25
IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Pbk Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Pbk
Synonyms Topk
Function Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).
Research Areas
Citations (0)

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Background

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).

References

Zykova, T.A., et al. Clin. Cancer Res. 12(23):6884-6893(2006)
Fujibuchi, T., et al. Dev. Growth Differ. 47(9):637-644(2005)
Blackshaw, S., et al. PLoS Biol. 2 (9), E247 (2004) :
Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
Easterday, M.C., et al. Dev. Biol. 264(2):309-322(2003)

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$ 295.00
$ 99.00
Cat# AP19430a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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