|Application ||WB, E|
|Other Accession||Q8CIN9, Q6ZQM0, NP_476519.1|
|Calculated MW||40514 Da|
|Antigen Region||265-294 aa|
|Other Names||E3 ubiquitin-protein ligase rififylin, 632-, Caspase regulator CARP2, Caspases-8 and -10-associated RING finger protein 2, CARP-2, FYVE-RING finger protein Sakura, Fring, RING finger and FYVE-like domain-containing protein 1, RING finger protein 189, RING finger protein 34-like, RFFL (HGNC:24821), RNF189, RNF34L|
|Target/Specificity||This RFFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human RFFL.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RFFL Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.|
|Cellular Location||Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Note=The FYVE-type zinc finger may mediate phosphatidylinositol phosphate- binding and control subcellular localization|
|Tissue Location||Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
RFFL has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Newton-Cheh, C., et al. Nat. Genet. 41(4):399-406(2009)
Liao, W., et al. Curr. Biol. 18(9):641-649(2008)
Yang, W., et al. Cell Cycle 7(5):670-682(2008)
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