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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RFFL Antibody(C-term)   

RFFL Antibody(C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RFFL Antibody(C-term) AP19448b
    RFFL Antibody (C-term) (Cat. #AP19448b) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RFFL antibody detected the RFFL protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WZ73
Other Accession Q8CIN9, Q6ZQM0, NP_476519.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40514 Da
Antigen Region 265-294 aa
Additional Information
Gene ID 117584
Other Names E3 ubiquitin-protein ligase rififylin, 632-, Caspase regulator CARP2, Caspases-8 and -10-associated RING finger protein 2, CARP-2, FYVE-RING finger protein Sakura, Fring, RING finger and FYVE-like domain-containing protein 1, RING finger protein 189, RING finger protein 34-like, RFFL (HGNC:24821), RNF189, RNF34L
Target/Specificity This RFFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human RFFL.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRFFL Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RFFL (HGNC:24821)
Synonyms RNF189, RNF34L
Function E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.
Cellular Location Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Note=The FYVE-type zinc finger may mediate phosphatidylinositol phosphate- binding and control subcellular localization
Tissue Location Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Research Areas
Citations (0)

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RFFL has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.


Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Newton-Cheh, C., et al. Nat. Genet. 41(4):399-406(2009)
Liao, W., et al. Curr. Biol. 18(9):641-649(2008)
Yang, W., et al. Cell Cycle 7(5):670-682(2008)

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$ 295.00
$ 99.00
Cat# AP19448b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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