|Application ||WB, E|
|Other Accession||Q00417, Q86WR9|
|Calculated MW||41642 Da|
|Antigen Region||3-36 aa|
|Other Names||Transcription factor 7, TCF-7, T-cell-specific transcription factor 1, T-cell factor 1, TCF-1, TCF7, TCF1|
|Target/Specificity||This TCF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-36 amino acids from the N-terminal region of human TCF7.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TCF7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T- lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.|
|Tissue Location||Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium|
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Provided below are standard protocols that you may find useful for product applications.
The T cell specific transcription factor TCF7 activates genes involved in immune regulation and thymocyte differentiation, and is a candidate locus for genetic susceptibility to type 1 diabetes.
Smit, L., et al., J. Biol. Chem. 279(17):17232-17240 (2004).
Ioannidis, V., et al., J. Immunol. 171(2):769-775 (2003).
Noble, J.A., et al., Diabetes 52(6):1579-1582 (2003).
Batlle, E., et al., Cell 111(2):251-263 (2002).
van de Wetering, M., et al., J. Biol. Chem. 267(12):8530-8536 (1992).
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