|Application ||WB, E|
|Calculated MW||56947 Da|
|Antigen Region||67-96 aa|
|Other Names||E3 ubiquitin-protein ligase TRIM22, 632-, 50 kDa-stimulated trans-acting factor, RING finger protein 94, Staf-50, Tripartite motif-containing protein 22, TRIM22, RNF94, STAF50|
|Target/Specificity||This TRIM22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-96 amino acids from the N-terminal region of human TRIM22.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRIM22 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Interferon-induced antiviral protein involved in cell innate immunity. The antiviral activity could in part be mediated by TRIM22-dependent ubiquitination of viral proteins. Plays a role in restricting the replication of HIV-1, encephalomyocarditis virus (EMCV) and hepatitis B virus (HBV). Acts as a transcriptional repressor of HBV core promoter. May have E3 ubiquitin-protein ligase activity.|
|Cellular Location||Cytoplasm. Nucleus. Nucleus speckle. Nucleus, Cajal body. Note=Localizes predominanltly into the nucleus, found in cytoplasm to some extent. Forms distinct nuclear bodies that undergo dynamic changes during cell cycle progression Nuclear bodies start to form in the early G0/G1 phase but become speckle-like in the S-phase and completely dispersed in mitosis 35% of TRIM22 nuclear bodies overlap or are found adjacent to Cajal bodies|
|Tissue Location||Strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary. Expressed at basal levels in other tissues.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects.
Kajaste-Rudnitski, A., et al. Amino Acids 39(1):1-9(2010)
Petersson, J., et al. Exp. Cell Res. 316(4):568-579(2010)
Ovsyannikova, I.G., et al. Hum. Genet. 127(2):207-221(2010)
Ovsyannikova, I.G., et al. J. Infect. Dis. 201(2):207-213(2010)
Gao, B., et al. Hepatology 50(2):424-433(2009)
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