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EIF4E Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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United States
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Ordering Information
Catalog # SizeAvailability Price  
AP1954a 400 µl
(40 western blots)
In Stock
$ 265.00email & fax$ 251.75online
Add to cart
AP1954a-ev 80 µl
(8 western blots)
In Stock
$ 95.00email & fax$ 90.25online
Add to cart
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EIF4E Antibody (N-term) - Product info

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary AccessionP06730
Other AccessionP48597, P63074, P29338, P63073, Q9N0T5, Q9DFS6
ReactivityHuman
PredictedMouse, Rat, Zebrafish, Bovine, Rabbit, Xenopus
Isotype Rabbit Ig
Calculated MW 25097 Da

EIF4E Antibody (N-term) - Additional info

Gene ID 1977
Other Names
EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
Target/Specificity
This EIF4E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human EIF4E.
Dilution
WB~~1:1000
IHC~~1:50~100
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
EIF4E Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

EIF4E Antibody (N-term) - Protein Information

Name EIF4E
Synonyms EIF4EL1, EIF4F
Function
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Cellular Location
Cytoplasm, P-body. Cytoplasm (By similarity)

EIF4E Antibody (N-term) - Related products

AM1852a: EIF4E Antibody (Ascites)

AM1852B: EIF4E Antibody

AP1954B: EIF4E Antibody

AP3474a: Phospho-EIF4E(S209) Antibody

BP3474a: Phospho-EIF4E-S209 Antibody Blocking Peptide

AJ1255a: eIF-4E Antibody

AJ1255b: eIF-4E Antibody (C-term)

AJ1255c: eIF-4E Antibody Phospho (pS209)

AE1011a: EIF4E Antibody (S209)

AE1011b: Phospho-EIF4E-S209 Antibody

AP50292: Phospho-eIF4E (Ser209) Antibody

AO1535a: EIF4E Antibody

EIF4E Antibody (N-term) - Research Areas

Application data

  • WB - EIF4E Antibody (N-term) AP1954a

    Western blot analysis of anti-EIF4E Antibody (N-term) Pab (AP1954a) in HL60 cell line lysates. EIF4E Antibody (N-term)(arrow) was detected using the purified Pab.

  • IHC - EIF4E Antibody (N-term) AP1954a

    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E antibody (N-term)(Cat.#AP1954a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • WB - EIF4E Antibody (N-term) AP1954a
  • IHC - EIF4E Antibody (N-term) AP1954a

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BACKGROUND

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.

REFERENCES

Rychlik,W., J. Biol. Chem. 262 (22), 10434-10437 (1987)
Dorfman,J., Genomics 9 (4), 785-788 (1991)
Pelletier,J., Genomics 10 (4), 1079-1082 (1991)
Whalen,S.G., J. Biol. Chem. 271 (20), 11831-11837 (1996)