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>   home   >   Products   >   Primary Antibodies   >   EIF4E Antibody (N-term)   

EIF4E Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EIF4E Antibody (N-term) AP1954a
    Western blot analysis of anti-EIF4E Antibody (N-term) Pab (AP1954a) in HL60 cell line lysates. EIF4E Antibody (N-term)(arrow) was detected using the purified Pab.
  • IHC-P - EIF4E Antibody (N-term) AP1954a
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E antibody (N-term)(Cat.#AP1954a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06730
Other Accession P48597, P63074, P29338, P63073, Q9N0T5, Q9DFS6
Reactivity Human
Predicted Zebrafish, Bovine, Mouse, Rabbit, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 25097 Da
Antigen Region 32-61 aa
Additional Information
Gene ID 1977
Other Names Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Target/Specificity This EIF4E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human EIF4E.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4E Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4E
Synonyms EIF4EL1, EIF4F
Function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Cellular Location Cytoplasm, P-body. Cytoplasm
Citations (0)

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eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.


Rychlik,W., J. Biol. Chem. 262 (22), 10434-10437 (1987)
Dorfman,J., Genomics 9 (4), 785-788 (1991)
Pelletier,J., Genomics 10 (4), 1079-1082 (1991)
Whalen,S.G., J. Biol. Chem. 271 (20), 11831-11837 (1996)

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$ 265.00
$ 95.00
Cat# AP1954a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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