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EIF4E Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1954a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
EIF4E Antibody (N-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | P06730 |
| Other Accession | P48597, P63074, P29338, P63073, Q9N0T5, Q9DFS6 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat, Rabbit, Xenopus, Zebrafish |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 25097 Da |
EIF4E Antibody (N-term) - Additional info | |
| Gene ID 1977 | |
| Other Names EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein | |
| Target/Specificity This EIF4E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39~69 amino acids from the N-terminal region of human EIF4E Antibody. | |
| Dilution WB~~1:250~500WB~~1:1000 IHC~~1:50-100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions EIF4E Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
EIF4E Antibody (N-term) - Protein Information | |
| Name EIF4E | |
| Synonyms EIF4EL1, EIF4F | |
| Function Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap | |
| Cellular Location Cytoplasm, P-body. | |
EIF4E Antibody (N-term) - Related products
AM1852a: EIF4E Antibody (Ascites)
AP1954a: EIF4E Antibody (N-term)
AP3474a: Phospho-EIF4E-S209 Antibody
RI11731: EIF4E predesign siRNA
DC03466: Human EIF4E cDNA Clone
BP3474a: Phospho-EIF4E-S209 Antibody Blocking Peptide
AJ1255b: eIF-4E Antibody (C-term)
AJ1255c: eIF-4E Antibody Phospho (pS209)
EIF4E Antibody (N-term) - Application data
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Western blot analysis of anti-EIF4E Antibody (N-term) Pab (AP1954a) in HL60 cell line lysates. EIF4E Antibody (N-term)(arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E antibody (N-term)(Cat.#AP1954a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
EIF4E Antibody (N-term) - Research Areas
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BACKGROUND
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.
REFERENCES
Rychlik,W., J. Biol. Chem. 262 (22), 10434-10437 (1987) Dorfman,J., Genomics 9 (4), 785-788 (1991) Pelletier,J., Genomics 10 (4), 1079-1082 (1991) Whalen,S.G., J. Biol. Chem. 271 (20), 11831-11837 (1996)
