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PIH1D1 Antibody(Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PIH1D1 Antibody(Center) AP19637c
    Anti-PIH1D1 Antibody (Center) at 1:1000 dilution + A375 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NWS0
Other Accession NP_060386.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 32363 Da
Antigen Region 163-192 aa
Additional Information
Gene ID 55011
Other Names PIH1 domain-containing protein 1, Nucleolar protein 17 homolog, PIH1D1, NOP17
Target/Specificity This PIH1D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-192 amino acids from the Central region of human PIH1D1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIH1D1 Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIH1D1
Synonyms NOP17
Function Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles (PubMed:17636026). Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre- rRNA transcription (PubMed:22368283, PubMed:24036451). Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1 (PubMed:20864032). Positively regulates the assembly and activity of the mTORC1 complex (PubMed:24036451).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q9CQJ2}.
Tissue Location Expressed at low levels in normal mammary epithelial cells (at protein level) (PubMed:24036451). Highest expression in lung, leukocyte and placenta. Expressed at lower levels in brain, prostate, colon, heart, small intestine, liver, ovary, pancreas, skeletal muscle, spleen, testis and thymus (PubMed:21078300)
Citations (0)
citation

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Background

PIH1D1 belongs to the PIH1 family.

References

Cloutier, P., et al. Methods 48(4):381-386(2009)
McKeegan, K.S., et al. Mol. Cell. Biol. 27(19):6782-6793(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Gonzales, F.A., et al. J. Mol. Biol. 346(2):437-455(2005)

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$ 365.00
$ 140.00
Cat# AP19637c
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