|Application ||WB, E|
|Other Accession||Q8VCK3, Q9NRH3, Q32KM1, P23330, P83888, P83887, Q0VCD2, NP_001061.2|
|Predicted||Bovine, Mouse, Rat, Xenopus|
|Calculated MW||51170 Da|
|Antigen Region||23-51 aa|
|Other Names||Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1, TUBG1, TUBG|
|Target/Specificity||This TUBG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human TUBG1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TUBG1 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):251-257(2010)
Alvarado-Kristensson, M., et al. Nat. Cell Biol. 11(9):1081-1092(2009)
Zhang, X., et al. J. Cell. Sci. 122 (PT 13), 2240-2251 (2009) :
Haren, L., et al. PLoS ONE 4 (6), E5976 (2009) :
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1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose