|Application ||WB, E|
|Other Accession||Q8WN55, NP_002810.1, NP_114367.1, NP_114368.1, NP_787041.1|
|Calculated MW||57221 Da|
|Antigen Region||26-55 aa|
|Other Names||Polypyrimidine tract-binding protein 1, PTB, 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I, PTBP1, PTB|
|Target/Specificity||This PTBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human PTBP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PTBP1 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).|
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Provided below are standard protocols that you may find useful for product applications.
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Kanda, T., et al. J. Viral Hepat. 17(9):618-623(2010)
Cobbold, L.C., et al. Oncogene 29(19):2884-2891(2010)
Verma, B., et al. J. Gen. Virol. 91 (PT 5), 1245-1255 (2010) :
Maynard, C.M., et al. J. Mol. Biol. 397(1):260-277(2010)
Xue, Y., et al. Mol. Cell 36(6):996-1006(2009)
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