- CITATIONS: 2
|Application ||WB, E|
|Calculated MW||68227 Da|
|Antigen Region||198-227 aa|
|Other Names||Condensin-2 complex subunit H2, Chromosome-associated protein H2, hCAP-H2, Kleisin-beta, Non-SMC condensin II complex subunit H2, NCAPH2, CAPH2|
|Target/Specificity||This NCAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the N-terminal region of human NCAPH2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NCAPH2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development (By similarity).|
|Cellular Location||Nucleus. Chromosome. Note=Distributed along the arms of chromosomes assembled in vivo and in vitro|
Provided below are standard protocols that you may find useful for product applications.
Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.
Ono,T., et al. Cell 115 (1), 109-121 (2003).
Schleiffer,A., et al. Mol. Cell 11 (3), 571-575 (2003).
Loftus, B.J., et al., Genomics 60(3):295-308 (1999).
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