|Application ||WB, E|
|Other Accession||P49816, Q61037, NP_000539.2|
|Calculated MW||200608 Da|
|Antigen Region||479-508 aa|
|Other Names||Tuberin, Tuberous sclerosis 2 protein, TSC2, TSC4|
|Target/Specificity||This TSC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-508 amino acids from the N-terminal region of human TSC2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TSC2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. May also play a role in microtubule-mediated protein transport. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5.|
|Cellular Location||Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes|
|Tissue Location||Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta|
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Provided below are standard protocols that you may find useful for product applications.
Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.
Slattery, M.L., et al. Carcinogenesis 31(9):1604-1611(2010)
Larson, Y., et al. J. Biol. Chem. 285(32):24987-24998(2010)
Mehta, M.S., et al. Breast Cancer Res. Treat. (2010) In press :
Mieulet, V., et al. Trends Mol Med 16(7):329-335(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
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