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ZNF219 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ZNF219 Antibody (N-term) AP1982a
    Western blot analysis of lysate from SH-SY5Y cell line, using ZNF219 Antibody (N-term)(Cat. #AP1982a). AP1982a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
    detail
  • WB - ZNF219 Antibody (N-term) AP1982a
    Western blot analysis of lysate from human heart tissue, using ZNF219 Antibody (N-term) (Cat. #AP1982a). AP1982a was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
    detail
  • WB - ZNF219 Antibody (N-term) AP1982a
    Western blot analysis of anti-ZNF219 Antibody (N-term) (Cat.#AP1982a) in 293 cell line lysates (35ug/lane).ZNF219(arrow) was detected using the purified Pab.
    detail
  • IHC-P - ZNF219 Antibody (N-term) AP1982a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with ZNF219 antibody (N-term) (Cat.#AP1982a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9P2Y4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 76877 Da
Antigen Region 154-183 aa
Additional Information
Gene ID 51222
Other Names Zinc finger protein 219, ZNF219
Target/Specificity This ZNF219 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human ZNF219.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZNF219 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZNF219
Function Transcriptional regulator (PubMed:14621294, PubMed:19549071). Recognizes and binds 2 copies of the core DNA sequence motif 5'-GGGGG- 3' (PubMed:14621294). Binds to the HMGN1 promoter and may repress HMGN1 expression (PubMed:14621294). Regulates SNCA expression in primary cortical neurons (PubMed:19549071). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with SOX9 (By similarity). Plays a role in chondrocyte differentiation (By similarity).
Cellular Location Nucleus
Tissue Location Ubiquitous..
Research Areas
Citations (0)
citation

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Background

ZNF219 is a member of the Kruppel-like zinc finger family that is involved in a diverse range of biological processes. ZNF219 is a 77-kDa nuclear protein containing nine sets of C2H2 zinc finger structures. It may be involved in transcriptional regulation, it functions as a transcriptional repressor for the HMGN1 promoter

References

Olsen,J.V., Cell 127 (3), 635-648 (2006)
Paces-Fessy,M., Biochem. J. 378 (PT 2), 353-362 (2004)
Sakai,T., DNA Res. 10 (4), 155-165 (2003)

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$ 365.00
$ 140.00
Cat# AP1982a
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