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PSMD4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PSMD4 Antibody (C-term) AP19888b
    PSMD4 Antibody (C-term) (Cat. #AP19888b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PSMD4 antibody detected the PSMD4 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P55036
Other Accession A2A3N6, NP_002801.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40737 Da
Antigen Region 300-328 aa
Additional Information
Gene ID 5710
Other Names 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, Antisecretory factor 1, AF, ASF, Multiubiquitin chain-binding protein, PSMD4, MCB1
Target/Specificity This PSMD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-328 amino acids from the C-terminal region of human PSMD4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMD4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMD4
Synonyms MCB1
Function Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
Research Areas
Citations (0)

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The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.


Elangovan, M., et al. Biochem. Biophys. Res. Commun. 396(2):425-428(2010)
Safadi, S.S., et al. J. Biol. Chem. 285(2):1424-1434(2010)
Zhang, N., et al. Mol. Cell 35(3):280-290(2009)
Kim, H.T., et al. EMBO J. 28(13):1867-1877(2009)
Gaurnier-Hausser, A., et al. Curr. Pharm. Des. 15(17):1937-1948(2009)

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2016-08-23 17:15:32
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Species tested
Application tested
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose
$ 295.00
$ 99.00
Cat# AP19888b
(40 western blots)
Availability: In Stock
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