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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RFWD2 Antibody (C-term)   

RFWD2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RFWD2 Antibody (C-term) AP19933b
    RFWD2 Antibody (C-term) (Cat. #AP19933b) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the RFWD2 antibody detected the RFWD2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8NHY2
Other Accession Q9R1A8, NP_071902.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 80474 Da
Antigen Region 672-701 aa
Additional Information
Gene ID 64326
Other Names E3 ubiquitin-protein ligase RFWD2, 632-, Constitutive photomorphogenesis protein 1 homolog, hCOP1, RING finger and WD repeat domain protein 2, RING finger protein 200, RFWD2, COP1, RNF200
Target/Specificity This RFWD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-701 amino acids from the C-terminal region of human RFWD2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRFWD2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RFWD2
Synonyms COP1, RNF200
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation.
Cellular Location Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles
Tissue Location Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart
Research Areas
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Background

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Kinyo, A., et al. J. Invest. Dermatol. 130(2):541-545(2010)
Li, D.Q., et al. Proc. Natl. Acad. Sci. U.S.A. 106(41):17493-17498(2009)
Kato, S., et al. J. Biol. Chem. 283(51):35464-35473(2008)

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$ 295.00
$ 99.00
Cat# AP19933b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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