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DMRTA2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - DMRTA2 Antibody (C-term) AP19969b
    Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)(Cat#AP19969b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.
    detail
  • WB - DMRTA2 Antibody (C-term) AP19969b
    DMRTA2 Antibody (C-term) (Cat. #AP19969b) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q96SC8
Other Accession A2A9A2, A6QQ94, NP_115486.1
Reactivity Human, Mouse, Zebrafish
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53356 Da
Antigen Region 487-515 aa
Additional Information
Gene ID 63950
Other Names Doublesex- and mab-3-related transcription factor A2, Doublesex- and mab-3-related transcription factor 5, DMRTA2, DMRT5
Target/Specificity This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDMRTA2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DMRTA2
Synonyms DMRT5
Function May be involved in sexual development.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00070}.
Tissue Location Expressed in testis..
Research Areas
Citations (0)
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Background

DMRTA2 may be involved in sexual development.

References

Ottolenghi, C., et al. Genomics 79(3):333-343(2002)

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$ 365.00
$ 140.00
Cat# AP19969b
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