|Application ||WB, E|
|Calculated MW||96023 Da|
|Antigen Region||541-570 aa|
|Other Names||Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95, PDCD6IP, AIP1, ALIX, KIAA1375|
|Target/Specificity||This PDCD6IP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-570 amino acids from the Central region of human PDCD6IP.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PDCD6IP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AIP1, ALIX, KIAA1375|
|Function||Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.|
|Cellular Location||Cytoplasm, cytosol. Melanosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Shi, X., et al. Biochem. J. 431(1):93-102(2010)
Irie, T., et al. Virology 405(2):334-341(2010)
Sette, P., et al. J. Virol. 84(16):8181-8192(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Inuzuka, T., et al. BMC Struct. Biol. 10, 25 (2010) :
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