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PUS10 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PUS10 Antibody (N-term) AP19991a
    PUS10 Antibody (N-term) (Cat. #AP19991a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PUS10 antibody detected the PUS10 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q3MIT2
Other Accession NP_653310.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 60244 Da
Antigen Region 52-78 aa
Additional Information
Gene ID 150962
Other Names Putative tRNA pseudouridine synthase Pus10, 5499-, Coiled-coil domain-containing protein 139, tRNA pseudouridine 55 synthase, Psi55 synthase, tRNA pseudouridylate synthase, tRNA-uridine isomerase, PUS10, CCDC139, DOBI
Target/Specificity This PUS10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-78 amino acids from the N-terminal region of human PUS10.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPUS10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PUS10
Synonyms CCDC139, DOBI
Function Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
Research Areas
Citations (0)

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Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM].


Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) :
McGovern, D.P., et al. Nat. Genet. 42(4):332-337(2010)
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Park, S.Y., et al. BMB Rep 42(8):511-515(2009)

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Cat# AP19991a
(40 western blots)
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