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CD3EAP Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CD3EAP Antibody (N-term) AP19997a
    CD3EAP Antibody (N-term) (Cat. #AP19997a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD3EAP antibody detected the CD3EAP protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O15446
Other Accession NP_036231.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54986 Da
Antigen Region 54-81 aa
Additional Information
Gene ID 10849
Other Names DNA-directed RNA polymerase I subunit RPA34, A345, Antisense to ERCC-1 protein, ASE-1, CD3-epsilon-associated protein, CAST, CD3E-associated protein, RNA polymerase I-associated factor PAF49, CD3EAP, ASE1, CAST, PAF49
Target/Specificity This CD3EAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-81 amino acids from the N-terminal region of human CD3EAP.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD3EAP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD3EAP
Synonyms ASE1, CAST, PAF49
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
Cellular Location Nucleus, nucleolus. Chromosome. Note=Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes
Citations (0)

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Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.

References

Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Vangsted, A.J., et al. Haematologica 94(9):1274-1281(2009)
Nissen, K.K., et al. Anticancer Drugs 20(3):174-178(2009)
Schierup, M.H., et al. BMC Med. Genet. 10, 20 (2009) :

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$ 295.00
$ 99.00
Cat# AP19997a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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