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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Osteocalcin Antibody (N-term)   

Osteocalcin Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Osteocalcin Antibody (N-term) AP2002a
    Anti-OSTC Antibody (C16) at 1:2000 dilution + MG-63 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Osteocalcin Antibody (N-term) AP2002a
    Western blot analysis of Osteocalcin (arrow) using rabbit polyclonal Osteocalcin Antibody (N-term) (Cat.#AP2002a).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BGLAP gene (Lane 2) (Origene Technologies).
  • IHC-P - Osteocalcin Antibody (N-term) AP2002a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02818
Other Accession NP_954642
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 10963 Da
Antigen Region 2-32 aa
Additional Information
Gene ID 632
Other Names Osteocalcin, Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein, BGLAP
Target/Specificity This Osteocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human Osteocalcin.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOsteocalcin Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Cellular Location Secreted.
Research Areas
Citations (0)

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Prior to the formation of calcified bone, noncollagenous proteins form in the extracellular bone matrix. Gamma-carboxyglutamic acid residues are formed by vitamin K, vitamin-D regulated calcium binding proteins containing residues of Gla. These residues are essential for the binding of calcium and constitue 1-2% of total bone protein. Osteocalcin itself binds strongly to apatite and calcium. Production of osteocalcin is expressed late in normal bone development and is characteristic of mature osteoblasts. Regular osteocalcin production has been shown to be linked to the p53 tumor suppressor gene. The p53 gene undergoes rearrangement in a high percentage of osteosarcomas, resulting in loss of its expression. The loss of p53 regulation inhibits further osteocalcin production. The absence of end-point differentiation in bone due to p53 rearrangements and lack of osteocalcin production may contribute to the maintenance of the tumorigenic phenotype in osteosarcomas.


Yamada, Y., et al., J. Clin. Endocrinol. Metab. 88(7):3372-3378 (2003). Gronthos, S., et al., J. Bone Miner. Res. 18(4):716-722 (2003). Yousfi, M., et al., Biochem. Biophys. Res. Commun. 297(3):641-644 (2002). Willis, D.M., et al., J. Biol. Chem. 277(40):37280-37291 (2002). Viereck, V., et al., J. Cell. Biochem. 86(2):348-356 (2002).

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$ 295.00
$ 99.00
Cat# AP2002a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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