|Application ||WB, E|
|Calculated MW||36080 Da|
|Antigen Region||297-325 aa|
|Other Names||C5a anaphylatoxin chemotactic receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5AR2, C5L2, GPR77|
|Target/Specificity||This GPR77 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-325 amino acids from the C-terminal region of human GPR77.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GPR77 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Frontal cortex, hippocampus, hypothalamus, pons and liver.|
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Provided below are standard protocols that you may find useful for product applications.
The anaphylatoxins C3a (see MIM 120700), C4a (see MIM 120810), and C5a are cationic fragments generated during the complement cascade that participate in host defense. In the case of inappropriate complement activation, anaphylatoxins may be involved in autoimmunity and sepsis. C5L2 is coexpressed with the C5a receptor, C5AR (C5R1; MIM 113995), on polymorphonuclear neutrophils and may modulate C5AR activity (Gerard et al., 2005 [PubMed 16204243]).
Bamberg, C.E., et al. J. Biol. Chem. 285(10):7633-7644(2010)
Cui, W., et al. Mol. Immunol. 46(16):3207-3217(2009)
Cui, W., et al. Mol. Immunol. 46(15):3086-3098(2009)
Scola, A.M., et al. Mol. Immunol. 46(6):1149-1162(2009)
Scola, A.M., et al. J. Biol. Chem. 282(6):3664-3671(2007)
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