ILF2 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q12905 |
Other Accession | Q7TP98, Q9CXY6, Q6NZ06, NP_004506.2 |
Reactivity | Human |
Predicted | Zebrafish, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 43062 Da |
Antigen Region | 84-112 aa |
Gene ID | 3608 |
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Other Names | Interleukin enhancer-binding factor 2, Nuclear factor of activated T-cells 45 kDa, ILF2, NF45 |
Target/Specificity | This ILF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the N-terminal region of human ILF2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ILF2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ILF2 |
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Synonyms | NF45 |
Function | Chromatin-interacting protein that forms a stable heterodimer with interleukin enhancer-binding factor 3/ILF3 and plays a role in several biological processes including transcription, innate immunity or cell growth (PubMed:18458058, PubMed:31212927). Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus. Together with ILF3, forms an RNA-binding complex that is required for mitotic progression and cytokinesis by regulating the expression of a cluster of mitotic genes. Mechanistically, competes with STAU1/STAU2-mediated mRNA decay (PubMed:32433969). Also plays a role in the inhibition of various viruses including Japanese encephalitis virus or enterovirus 71. |
Cellular Location | Nucleus, nucleolus. Cytoplasm. Nucleus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
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Provided below are standard protocols that you may find useful for product applications.
Background
Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.
References
Karmakar, S., et al. EMBO J. 29(19):3260-3271(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Graber, T.E., et al. Cell Death Differ. 17(4):719-729(2010)
Kiesler, P., et al. J. Biol. Chem. 285(11):8256-8267(2010)
Sakamoto, S., et al. Mol. Cell. Biol. 29(13):3754-3769(2009)
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