|Application ||WB, E|
|Other Accession||B5DFI8, Q921J4, Q1RML1, A1L3K1, NP_055316.2|
|Predicted||Xenopus, Bovine, Mouse, Rat|
|Calculated MW||23845 Da|
|Antigen Region||183-210 aa|
|Other Names||Ubiquitin-conjugating enzyme E2 S, E2-EPF, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-protein ligase S, UBE2S, E2EPF|
|Target/Specificity||This UBE2S antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the C-terminal region of human UBE2S.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE2S Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.
Wu, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1355-1360(2010)
Garnett, M.J., et al. Nat. Cell Biol. 11(11):1363-1369(2009)
Williamson, A., et al. Proc. Natl. Acad. Sci. U.S.A. 106(43):18213-18218(2009)
Chen, M.F., et al. J. Mol. Med. 87(3):307-320(2009)
Lim, J.H., et al. J. Cell. Biochem. 105(4):1117-1127(2008)
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