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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Mouse Irf3 Antibody (N-term)   

Mouse Irf3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Irf3 Antibody (N-term) AP20073a
    MOUSE Irf3 Antibody (N-term) (Cat. #AP20073a) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the MOUSE Irf3 antibody detected the MOUSE Irf3 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P70671
Other Accession Q764M6, Q4JF28, NP_058545.1
Reactivity Mouse
Predicted Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46852 Da
Antigen Region 46-72 aa
Additional Information
Gene ID 54131
Other Names Interferon regulatory factor 3, IRF-3, Irf3
Target/Specificity This Mouse Irf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids from the N-terminal region of mouse Irf3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Irf3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Irf3
Function Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm (By similarity).
Research Areas
Citations (0)

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Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains (By similarity).


Marichal, T., et al. J. Allergy Clin. Immunol. 126(4):836-844(2010)
Menachery, V.D., et al. J. Virol. 84(19):9685-9694(2010)
Carrigan, S.O., et al. J. Immunol. 185(6):3602-3609(2010)
Wang, J., et al. J. Immunol. 185(3):1720-1729(2010)
Farlik, M., et al. Immunity 33(1):25-34(2010)

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$ 295.00
$ 99.00
Cat# AP20073a
(40 western blots)
Availability: In Stock
Bulk Size
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