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HOXC10 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - HOXC10 Antibody (C-term) AP20089b
    Fluorescent image of Hela cell stained with HOXC10 Antibody (C-term)(Cat#AP20089b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXC10 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).HOXC10 immunoreactivity is localized to Nucleus significantly.
  • WB - HOXC10 Antibody (C-term) AP20089b
    HOXC10 Antibody (C-term) (Cat. #AP20089b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HOXC10 antibody detected the HOXC10 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession Q9NYD6
Other Accession P31257, NP_059105.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38073 Da
Antigen Region 224-250 aa
Additional Information
Gene ID 3226
Other Names Homeobox protein Hox-C10, Homeobox protein Hox-3I, HOXC10, HOX3I
Target/Specificity This HOXC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-250 amino acids from the C-terminal region of human HOXC10.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHOXC10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HOXC10
Synonyms HOX3I
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Cellular Location Nucleus.
Citations (0)

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Background

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The protein level is controlled during cell differentiation and proliferation, which may indicate this protein has a role in origin activation.

References

Hwang, J.H., et al. Cloning Stem Cells 11(2):269-279(2009)
Zhai, Y., et al. Cancer Res. 67(21):10163-10172(2007)
Gabellini, D., et al. EMBO J. 22(14):3715-3724(2003)
Kosaki, K., et al. Teratology 65(2):50-62(2002)
Sandrock, B., et al. J. Biol. Chem. 276(38):35328-35333(2001)

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$ 295.00
$ 99.00
Cat# AP20089b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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