- CITATIONS: 4
|Application ||IHC-P, E|
|Calculated MW||63368 Da|
|Antigen Region||1-30 aa|
|Other Names||5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE|
|Target/Specificity||This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CD73 (NT5E).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD73 (NT5E) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
Provided below are standard protocols that you may find useful for product applications.
Ecto-5-prime-nucleotidase catalyzes conversion at neutral pH of purine 5-prime mononucleotides to nucleosides. The enzyme consists of a dimer of 2 identical 70 kD subunits tethered by a glycosyl phosphatidyl inositol moeity to the exterior plasma membrane surface. The enzyme, a marker of lymphocyte differentiation is associated in deficiency with a variety of immunodeficiency diseases. The preferred substrate is AMP. The NT5 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations.
Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003).
Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002).
Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990).
Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988).
Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).
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