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T Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - T Antibody (N-term) AP20187A
    T Antibody (N-term) (Cat. #AP20187a) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the T antibody detected the T protein (arrow).
  • IF - T Antibody (N-term) AP20187A
    Fluorescent confocal image of Hela cell stained with T Antibody (N-term)(Cat#AP20187a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with T primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). T immunoreactivity is localized to Nucleus significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15178
Other Accession P20293, P79777, NP_003172.1
Reactivity Human
Predicted Mouse, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 47443 Da
Antigen Region 15-43 aa
Additional Information
Gene ID 6862
Other Names Brachyury protein, Protein T, T
Target/Specificity This T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human T.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name T
Function Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
Cellular Location Nucleus
Tissue Location Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level)
Research Areas
Citations (0)

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The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.


Jambhekar, N.A., et al. Arch. Pathol. Lab. Med. 134(8):1181-1187(2010)
Docherty, S.J., et al. BMC Genet. 11, 61 (2010) :
Yang, X.R., et al. Nat. Genet. 41(11):1176-1178(2009)
Oakley, G.J., et al. Mod. Pathol. 21(12):1461-1469(2008)
Bondue, A., et al. Cell Stem Cell 3(1):69-84(2008)

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Cat# AP20187A
(40 western blots)
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