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T Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | O15178 |
| Other Accession | P20293, P79777, NP_003172.1 |
| Reactivity | Human |
| Predicted | Chicken, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 47443 Da |
| Antigen Region | 15-43 aa |
| Gene ID | 6862 |
|---|---|
| Other Names | Brachyury protein, Protein T, T |
| Target/Specificity | This T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human T. |
| Dilution | IF~~1:25 WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | T Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TBXT (HGNC:11515) |
|---|---|
| Synonyms | T |
| Function | Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site. |
| Cellular Location | Nucleus |
| Tissue Location | Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
References
Jambhekar, N.A., et al. Arch. Pathol. Lab. Med. 134(8):1181-1187(2010)
Docherty, S.J., et al. BMC Genet. 11, 61 (2010) :
Yang, X.R., et al. Nat. Genet. 41(11):1176-1178(2009)
Oakley, G.J., et al. Mod. Pathol. 21(12):1461-1469(2008)
Bondue, A., et al. Cell Stem Cell 3(1):69-84(2008)
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