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NSF Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NSF Antibody (C-term) AP20197B
    All lanes : Anti-NSF Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - NSF Antibody (C-term) AP20197B
    NSF Antibody (C-term) (Cat. #AP20197b) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the NSF antibody detected the NSF protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P46459
Other Accession Q9QUL6, P46460, P18708, NP_006169.2
Reactivity Human, Mouse
Predicted Hamster, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 82594 Da
Antigen Region 668-696 aa
Additional Information
Gene ID 4905
Other Names Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, NSF
Target/Specificity This NSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 668-696 amino acids from the C-terminal region of human NSF.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNSF Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NSF
Function Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

References

Saus, E., et al. J Psychiatr Res 44(14):971-978(2010)
Hamza, T.H., et al. Nat. Genet. 42(9):781-785(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Parashuraman, S., et al. FEBS Lett. 584(6):1251-1256(2010)
Simon-Sanchez, J., et al. Nat. Genet. 41(12):1308-1312(2009)

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$ 295.00
$ 99.00
Cat# AP20197B
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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