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ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220) AP2019d
    All lanes : Anti-Ascl1 (Human C-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220) AP2019d
    Western blot analysis of anti-Ascl1 Antibody (C-term D220) (Cat. #AP2019d) in Hela cell line lysates (35ug/lane). Ascl1(arrow) was detected using the purified Pab (1:160 dilution).
  • WB - ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220) AP2019d
    Western blot analysis of Ascl1 (arrow) using rabbit polyclonal Ascl1 Antibody (Human C-term) (Cat.#AP2019d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ascl1 gene (Lane 2) (Origene Technologies).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P50553
Other Accession Q90259, P19359, Q02067, NP_004307
Reactivity Human
Predicted Mouse, Rat, Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 25454 Da
Additional Information
Gene ID 429
Other Names Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46, ASCL1, ASH1, BHLHA46, HASH1
Target/Specificity This ASCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ASCL1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsASCL1 (Achaete-scute homolog 1) Antibody (C-term D220) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ASCL1
Synonyms ASH1, BHLHA46, HASH1
Function Transcription factor that controls transcriptional expression of its target genes by binding to the E box (5'-CANNTG- 3'). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Essential for the generation of olfactory and autonomic neurons (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)

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ACSL1, alternatively titled Hash1 or Mash1, is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. ACSL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. The protein is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers. The presence of a CAG repeat in the gene suggests it may also play a role in tumor formation.


Sriuranpong, V., et al., Mol. Cell. Biol. 22(9):3129-3139 (2002).
Westerman, B.A., et al., Clin. Cancer Res. 8(4):1082-1086 (2002).
Chen, H., et al., Cell Growth Differ. 8(6):677-686 (1997).
Borges, M., et al., Nature 386(6627):852-855 (1997).
Renault, B., et al., Genomics 30(1):81-83 (1995).

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$ 295.00
Cat# AP2019d
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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