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Neurogenin3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Neurogenin3 Antibody (N-term) AP2024A
    All lanes : Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: LNCap whole cell lysate Lane 3: PANC-1 whole cell lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - Neurogenin3 Antibody (N-term) AP2024A
    Neurogenin3 Antibody (N-term) (Cat. #AP2024a) immunohistochemistry analysis in formalin fixed and paraffin embedded human large intestine followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Neurogenin3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - Neurogenin3 Antibody (N-term) AP2024A
    Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) (Cat#AP2024a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y4Z2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23077 Da
Antigen Region 40-69 aa
Additional Information
Gene ID 50674
Other Names Neurogenin-3, NGN-3, Class A basic helix-loop-helix protein 7, bHLHa7, Protein atonal homolog 5, NEUROG3, ATOH5, BHLHA7, NGN3
Target/Specificity This Neurogenin3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human Neurogenin3.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurogenin3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ATOH5, BHLHA7, NGN3
Function Acts as a transcriptional regulator. Together with NKX2- 2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981}
Research Areas
Citations (0)

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Neurogenin-3 belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm. Neurogenin-3 also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Defects in NEUROG3 are the cause of congenital malabsorptive diarrhea 4 (DIAR4). DIAR4 is an autosomal recessive disorder characterized by generalized malabsorption and a paucity of enteroendocrine cells.


Heremans, Y., et al., J. Cell Biol. 159(2):303-312 (2002). Sommer, L., et al., Mol. Cell. Neurosci. 8(4):221-241 (1996).

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Cat# AP2024A
(40 western blots)
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