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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RAB27A Antibody (C-term)   

RAB27A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RAB27A Antibody (C-term) AP20264b
    Western blot analysis of lysate from K562 cell line, using RAB27A Antibody (C-term)(Cat. #AP20264b). AP20264b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P51159
Other Accession Q99P74, Q99P58, P23640, Q4LE85, Q9ERI2, NP_004571.2
Reactivity Human
Predicted Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24868 Da
Antigen Region 136-164 aa
Additional Information
Gene ID 5873
Other Names Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27
Target/Specificity This RAB27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the C-terminal region of human RAB27A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB27A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB27A
Synonyms RAB27
Function Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Cellular Location Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). Localizes to endosomal exocytic vesicles (PubMed:17237785).
Tissue Location Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
Research Areas
Citations (0)

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Background

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

References

Ohbayashi, N., et al. Pigment Cell Melanoma Res 23(3):365-374(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Meeths, M., et al. Pediatr Blood Cancer 54(4):563-572(2010)
Chattopadhyay, I., et al. Mutat. Res. 696(2):130-138(2010)
Wood, S.M., et al. Blood 114(19):4117-4127(2009)

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$ 295.00
$ 99.00
Cat# AP20264b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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