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CPSF6 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CPSF6 Antibody (N-term) AP20278a
    Anti-CPSF6 Antibody (N-term) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CPSF6 Antibody (N-term) AP20278a
    CPSF6 Antibody (N-term) (Cat. #AP20278a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CPSF6 antibody detected the CPSF6 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16630
Other Accession Q6DDW4, Q6NVF9, Q6NWC6, Q5ZL34, Q0P5D2, NP_008938.2
Reactivity Human
Predicted Bovine, Chicken, Zebrafish, Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 59210 Da
Antigen Region 23-51 aa
Additional Information
Gene ID 11052
Other Names Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, CFIm68, CPSF 68 kDa subunit, Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7, CPSF6, CFIM68
Target/Specificity This CPSF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human CPSF6.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCPSF6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CPSF6
Synonyms CFIM68
Function Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.
Cellular Location Nucleus. Note=In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles
Citations (0)

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The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.


Lee, K., et al. Cell Host Microbe 7(3):221-233(2010)
Ruepp, M.D., et al. Mol. Biol. Cell 20(24):5211-5223(2009)
Xin, X., et al. Genome Res. 19(7):1262-1269(2009)
Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008)

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$ 295.00
$ 99.00
Cat# AP20278a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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