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Mouse p27Kip1 Antibody (C-term T197)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse p27Kip1 Antibody (C-term T197) AP20325b
    Anti-Mouse p27Kip1 Antibody (C-term T197) at 1:1000 dilution + C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • WB - Mouse p27Kip1 Antibody (C-term T197) AP20325b
    Mouse p27Kip1 Antibody (C-term T197) (Cat. #AP20325b) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the (mouse) p27Kip1 antibody detected the (mouse) p27Kip1 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P46414
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22193 Da
Antigen Region 175-197 aa
Additional Information
Gene ID 12576
Other Names Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b
Target/Specificity This Mouse p27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-197 amino acids from the C-terminal region of mouse p27Kip1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse p27Kip1 Antibody (C-term T197) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cdkn1b
Function Important regulator of cell cycle progression (PubMed:8033213, PubMed:12972555). Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (By similarity). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes (PubMed:8033213). Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Cellular Location Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-197, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 (By similarity) Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (PubMed:20228253). {ECO:0000250, ECO:0000269|PubMed:20228253}
Research Areas
Citations (0)
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Background

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E-and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of cyclin D-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

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$ 365.00
$ 140.00
Cat# AP20325b
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