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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Mouse p27Kip1 Antibody (C-term T197)   

Mouse p27Kip1 Antibody (C-term T197)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse p27Kip1 Antibody (C-term T197) AP20325b
    Anti-Mouse p27Kip1 Antibody (C-term T197) at 1:1000 dilution + C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Mouse p27Kip1 Antibody (C-term T197) AP20325b
    Mouse p27Kip1 Antibody (C-term T197) (Cat. #AP20325b) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the (mouse) p27Kip1 antibody detected the (mouse) p27Kip1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P46414
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22193 Da
Antigen Region 175-197 aa
Additional Information
Gene ID 12576
Other Names Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b
Target/Specificity This Mouse p27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-197 amino acids from the C-terminal region of mouse p27Kip1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse p27Kip1 Antibody (C-term T197) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cdkn1b
Function Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of cyclin D-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Cellular Location Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-197, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser- 10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 (By similarity). Colocalizes at the endosome with SNX6; this leads to lysosomal degradation
Research Areas
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Background

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E-and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of cyclin D-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

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$ 295.00
$ 99.00
Cat# AP20325b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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