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PDX1 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2034a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
PDX1 Antibody (N-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | O00330 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 54122 Da |
PDX1 Antibody (N-term) - Additional info | |
| Gene ID 8050 | |
| Other Names PDHX; PDX1; Pyruvate dehydrogenase protein X component, mitochondrial; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; E3-binding protein; Lipoyl-containing pyruvate dehydrogenase complex component X; proX | |
| Target/Specificity This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-terminal region of human PDX1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PDX1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PDX1 Antibody (N-term) - Protein Information | |
| Name PDHX | |
| Synonyms PDX1 | |
| Function Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex | |
| Cellular Location Mitochondrion matrix. | |
PDX1 Antibody (N-term) - Related products
AP2034a: PDX1 Antibody (N-term)
AP2034b: PDX1 Antibody (C-term)
AP3628a: Phospho-PDX1-pS66 Antibody
DC02916: Human PDHX cDNA Clone
LY11556a: PDHX Over-expression Lysate
BP2034a: PDX1 Antibody (N-term) Blocking Peptide
BP2034b: PDX1 Antibody (C-term) Blocking Peptide
BP2034d: PDX1 Antibody (T11) Blocking Peptide
PDX1 Antibody (N-term) - Application data
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Western blot analysis of hPDX1-C9 (Cat. #AP2034a) in NCI-H460 cell line lysates (35ug/lane). PDX1 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PDX1 Antibody (N-term) - Research Areas
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BACKGROUND
PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.
REFERENCES
Ling, M., et al., Hum. Mol. Genet. 7(3):501-505 (1998). Aral, B., et al., Am. J. Hum. Genet. 61(6):1318-1326 (1997). Harris, R.A., et al., J. Biol. Chem. 272(32):19746-19751 (1997). Yu, W., et al., Genome Res. 7(4):353-358 (1997).
