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PRS4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - PRS4 Antibody (C-term) AP20440b
    Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody (C-term)(Cat#AP20440b). AP20440b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IF - PRS4 Antibody (C-term) AP20440b
    Fluorescent image of Hela cells stained with PRS4 Antibody (C-term)(Cat#AP20440b). AP20440b was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • IHC-P - PRS4 Antibody (C-term) AP20440b
    Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody (C-term)(Cat#AP20440b). AP20440b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IF - PRS4 Antibody (C-term) AP20440b
    Fluorescent image of Hela cells stained with PRS4 Antibody (C-term)(Cat#AP20440b). AP20440b was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - PRS4 Antibody (C-term) AP20440b
    PRS4 Antibody (C-term) (Cat. #AP20440b) western blot analysis in A549,Hela cell line and mouse heart and lung,rat lung tissue lysates (35ug/lane).This demonstrates the PRS4 antibody detected the PRS4 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession P62191
Other Accession P62193, P62192
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49185 Da
Antigen Region 408-436 aa
Additional Information
Gene ID 5700
Other Names 26S protease regulatory subunit 4, P26s4, 26S proteasome AAA-ATPase subunit RPT2, Proteasome 26S subunit ATPase 1, PSMC1
Target/Specificity This PRS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of human PRS4.
Dilution IHC-P~~1:25
IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRS4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMC1
Function The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Cellular Location Cytoplasm. Nucleus.
Research Areas
Citations (0)

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Background

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

References

Dubiel W., et al. J. Biol. Chem. 267:22699-22702(1992).
Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Matilla A., et al. Hum. Mol. Genet. 10:2821-2831(2001).
Brill L.M., et al. Anal. Chem. 76:2763-2772(2004).
Katiyar S., et al. Proc. Natl. Acad. Sci. U.S.A. 101:13774-13779(2004).

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$ 295.00
$ 99.00
Cat# AP20440b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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