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UBE3C Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - UBE3C Antibody (Center) AP20457c
    Western blot analysis of lysates from Hela cell line, mouse brain tissue lysate, HepG2 cell line (from left to right), using UBE3CAntibody(Center)(Cat. #AP20457c). AP20457c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - UBE3C Antibody (Center) AP20457c
    UBE3C Antibody (Center) (Cat. #AP20457c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE3C antibody detected the UBE3C protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q15386
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 123923 Da
Antigen Region 549-578 aa
Additional Information
Gene ID 9690
Other Names Ubiquitin-protein ligase E3C, 632-, HectH2, UBE3C, KIAA0010, KIAA10
Target/Specificity This UBE3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-578 amino acids from the Central region of human UBE3C.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE3C Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE3C
Synonyms KIAA0010, KIAA10
Function E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
Cellular Location Nucleus.
Tissue Location Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas
Research Areas
Citations (0)

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Background

E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'-or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.

References

Nomura N., et al. DNA Res. 1:27-35(1994).
Hillier L.W., et al. Nature 424:157-164(2003).
Scherer S.W., et al. Science 300:767-772(2003).
Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
You J., et al. J. Biol. Chem. 276:19871-19878(2001).

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$ 295.00
$ 99.00
Cat# AP20457c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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