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PPP2R2A Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - PPP2R2A Antibody (N-term) AP20472a
    Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term)(Cat#AP20472A). AP20472A was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IHC-P - PPP2R2A Antibody (N-term) AP20472a
    Immunohistochemical analysis of paraffin-embedded H. kidney section using PPP2R2A Antibody (N-term)(Cat#AP20472A). AP20472A was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IF - PPP2R2A Antibody (N-term) AP20472a
    Fluorescent image of Hela cells stained with PPP2R2A Antibody (N-term)(Cat#AP20472A). AP20472A was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - PPP2R2A Antibody (N-term) AP20472a
    PPP2R2A Antibody (N-term) (Cat. #AP20472a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PPP2R2A antibody detected the PPP2R2A protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession P63151
Other Accession P36876, P63150, Q29090, Q6P1F6, Q4R7Z4
Reactivity Human
Predicted Monkey, Mouse, Pig, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51692 Da
Antigen Region 43-71 aa
Additional Information
Gene ID 5520
Other Names Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform, PP2A subunit B isoform B55-alpha, PP2A subunit B isoform PR55-alpha, PP2A subunit B isoform R2-alpha, PP2A subunit B isoform alpha, PPP2R2A
Target/Specificity This PPP2R2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human PPP2R2A.
Dilution IHC-P~~1:25
IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPP2R2A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP2R2A
Function The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Tissue Location Expressed in all tissues examined.
Research Areas
Citations (0)

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Background

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

References

Mayer R.E., et al. Biochemistry 30:3589-3597(1991).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Nusbaum C., et al. Nature 439:331-335(2006).
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Li H.H., et al. EMBO J. 26:402-411(2007).

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Cat# AP20472a
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(40 western blots)
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