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SOX2 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2048d | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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SOX2 Antibody (N-term) - Product info | |
| Application | WB, IHC, IF, FC
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| Primary Accession | P48431 |
| Other Accession | Q6EJB7, O42569, P48432, Q6P0E1, P48430, Q2PG84, P53783, O00570, O57401, Q2Z1R2, Q6DGL6 |
| Reactivity | Human |
| Predicted | Chicken, Mouse, Xenopus, Zebrafish |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 34310 Da |
SOX2 Antibody (N-term) - Additional info | |
| Gene ID 6657 | |
| Other Names SOX2; Transcription factor SOX-2 | |
| Target/Specificity This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~126 amino acids from the N-terminal region of human SOX2. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SOX2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SOX2 Antibody (N-term) - Protein Information | |
| Name SOX2 | |
| Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity) | |
| Cellular Location Nucleus. | |
SOX2 Antibody (N-term) - Related products
AM2048b: SOX2 Antibody (Ascites)
AP2048b: SOX2 Antibody (C-term)
AP2048c: SOX2 Antibody (Center)
AP2048d: SOX2 Antibody (N-term)
AP3651a: Phospho-SOX2-S83 Antibody
AP3730a: Phospho-SOX2- Y108 Antibody
AP3736a: Phospho-Sox2- pS249 Antibody
AP3737a: phospho-Sox2- pS250 Antibody
AP3742a: phospho-Sox2- S251 Antibody
AP3750a: phospho-Sox2- S246 Antibody
AP5651a: Sox2-pS246-pS249-pS250--pS251 Antibody (C-term)
DC03709: Human SOX2 cDNA Clone
LY12065a: SOX2 Over-expression Lysate
BP2048b: SOX2 Antibody (C-term) Blocking Peptide
BP2048c: SOX2 Antibody (Center) Blocking Peptide
BP2048d: SOX2 Antibody (N-term) Blocking Peptide
BP2048e: SOX2 Antibody (C265) Blocking Peptide
BP2048g: SOX2 Antibody (A30) Blocking Peptide
BP3651a: Phospho-SOX2-S83 Antibody Blocking Peptide
BP3730a: Phospho-SOX2-Y108 Antibody Blocking peptide
BP5651a: Sox2-pS246-pS249-pS250--pS251 Antibody (C-term) Blocking peptide
SOX2 Antibody (N-term) - Application data
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Western blot analysis of SOX2 Antibody (N-term) in 293 cell line lysates (35ug/lane). SOX2(arrow) was detected using the purified Pab.
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Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (N-term) (Cat.#AP2048d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 antibody (N-term) (Cat.#AP2048d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Immunofluorescence analysis of SOX2 Antibody (N-term) (Cat.#AP2048d) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
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Flow cytometric analysis of NCI-H292 cells using SOX2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SOX2 Antibody (N-term) - Research Areas
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BACKGROUND
The intronless gene for SOX2 encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
REFERENCES
Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003). Wiebe, M.S., et al., J. Biol. Chem. 278(20):17901-17911 (2003). Fantes, J., et al., Nat. Genet. 33(4):461-463 (2003). Schepers, G.E., et al., Dev. Cell 3(2):167-170 (2002). Kamachi, Y., et al., Trends Genet. 16(4):182-187 (2000).
