|Application ||WB, IF, FC, E|
|Other Accession||O42569, P48432, P48430, P54231|
|Predicted||Chicken, Mouse, Sheep, Xenopus|
|Calculated MW||34310 Da|
|Antigen Region||251-284 aa|
|Other Names||Transcription factor SOX-2, SOX2|
|Target/Specificity||This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-284 amino acids from human SOX2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SOX2 Antibody (C265) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
References for protein:
1.Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003).
2.Wiebe, M.S., et al., J. Biol. Chem. 278(20):17901-17911 (2003).
3.Fantes, J., et al., Nat. Genet. 33(4):461-463 (2003).
4.Schepers, G.E., et al., Dev. Cell 3(2):167-170 (2002).
5.Kamachi, Y., et al., Trends Genet. 16(4):182-187 (2000).
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
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